2'-O-Me-C(Bz) Phosphoramidite: Modified Nucleotide for Stable Oligonucleotide Synthesis | |
2'-O-Me-C(Bz) Phosphoramidite (CAS: 110764-78-8) is a modified nucleotide used in oligonucleotide synthesis. It features a 2'-O-methyl (Me) modification and a benzoyl (Bz) group on cytidine (C), enhancing the stability and functionality of RNA sequences. Key Features: 2'-O-Methyl Group: Protects the 2' hydroxyl, improving stability against nucleases and enhancing overall resistance to degradation. Benzoyl Group: Stabilizes the amine group at the N4 position of cytidine, increasing the effectiveness of nucleotide incorporation. Applications: Oligonucleotide Synthesis: Ideal for synthesizing stable RNA sequences with modified bases. Gene Expression Studies: Used in probes and primers for analyzing RNA expression and regulation. Antisense Oligonucleotides: Provides enhanced stability for therapeutic applications targeting RNA. Diagnostics: Supports the development of RNA-based diagnostic assays. 2'-O-Me-C(Bz) Phosphoramidite is essential for creating modified oligonucleotides in research, diagnostics, and therapeutic development. | |
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